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List of posters presented at the 2005 meeting in Uppsala, Sweden
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801 Multiple frequency detection of antibody-antigen interactions via a novel acoustic wave biosensor.
Kioupritzi E., Araya-Kleinsteuber, B., Roque, A.C.A., Stevenson, A.C., Lowe, C.R.
Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, UK CB2 1QT. Tel: +44 (0) 1223 334152, Fax: +44 (0) 1223 334162, email: ek283@cam.ac.uk
802 Critical Interactions for Anisotropic Adsorption of Particles to Polymer Grafted Surfaces
Hans-Olof Johansson*1 and Fernando Barroso da Silva2, 1 Department of Biochemistry, Lund University, P.O.Box 124, S-22100 Lund, Sweden. Tel +46-46-2228189; Fax +46-46-2224534; e-mail Hans-Olof.Johansson@biokem.lu.se, 2 Faculty of Pharmaceutical Sciences at Ribeirao Preto, University of Sao Paulo-USP. Av. Do Café, s/no-FCFRP/Bloco A-sala 133, BR-14040-903, Ribeirao Preto SP, Brazil. Tel +55(16)6024219; Fax +55(16)6332960, e-mail: fernando@fcfrp.usp.br
* Corresponding author
803 Stereoselective recognition of positively or negatively charged chiral and achiral molecules by transferrin
Ferenc Kilár1, Petronella Kuti1, Csenge Sági1, Nándor Sánta2, Vlad Chindea2, Béla Tőkés2, 1Institute of Bioanalysis, Faculty of Medicine, University of Pécs Szigeti út 12., H-7624 Pécs, Hungary, 2University of Medicine and Pharmacy, Targu Mures, Romania
804 Predicting protein retention time in hydrophobic interaction chromatography
M.E. Lienqueo1, A. Mahn2 , J.C. Salgado1, I Rapaport3 and J.A. Asenjo1
1Centre for Biochemical Engineering and Biotechnology, Department of Chemical and Biotechnology Engineering, University of Chile, Beauchef 861,Santiago, Chile, e-mail mlienque@ing.uchile.cl 2Center for Molecular Cell Studies, Institute for Biomedical Sciences, University of Chile. 3Centre for Mathematical Modeling, University of Chile.
805 Affinity measurement of carbohydrate binding to recombinant Aleuria aurantia
lectin using fluorescence spectroscopy

Johan Olausson1, Lena Tibell1, Bengt-Harald Jonsson2, Peter Påhlsson1, 1Department of Biomedicine and Surgery, Division of Cell Biology, Linköping University, SE-581 85 Linköping. 2Molecular Biotechnology/IFM, Linköping University, SE-58183 Linkoping, Sweden.
806 Phosphatase Inhibitors – A Biacore Approach to Study Small Molecule Inhibitor
Interactions with Protein Phosphatases

Peter Stenlund, Åsa Frostell-Karlsson, Annika Remaeus, Åsa Edström and Olof Karlsson, Systems and Applications, Biacore AB, Uppsala, Sweden.
807 Bioanalytical applications of a non contact acoustic sensor
Araya-Kleinsteuber, B., Stevenson A.C., Roque, A.C.A., Kioupritzi, E. & Lowe, C.R.
Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, UK CB2 1QT. Tel: +44 (0) 1223 334152 Fax: +44 (0) 1223 334162, email: ba239@cam.ac.uk
808 Immobilized Metal Affinity Chromatography of Nucleic Acids
Ajish Potty1, Yuchun Fu1, Tony Cano1, Sindhu Balan2, George E. Fox2, and Richard C. Willson1,2, 1Department of Chemical Engineering, University of Houston, 4800 Calhoun Rd, Houston, TX 77204-4004, 2Department of Biology and Biochemistry, University of Houston, 4800 Calhoun Rd, Houston, TX 77204-5934
809 A label-free continuous fluorescence-based immunosensor
Henrik A. Engström1, Per Ola Andersson2 and Sten Ohlson1, 1Department of Chemistry and Biomedical Sciences, University of Kalmar, SE-391 82 Kalmar, Sweden. 2Swedish Defence Research Agency, Division of NBC-Defence, SE-901 82 Umeå, Sweden.
810 A TiO2-Binding Protein from Rhodococcus Strain GIN-1 (NCIMB 40340) – Mode of protein-oxide interaction
Gideon Fleminger, Jenny Komanovsky, Irene Brudo and Golan Gertler, Department of Molecular Microbiology and Biotechnology, George Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
811 Thermodynamic Dissection of Association and Dissociation in Interaction Between
Lysozyme and Monoclonal Antibodies

A. Zhukov1, T. Nakanishi2, K. Tsumoto2, I. Kumagai2, K. Andersson1, H. Nordin1, C Wass1,Å Edström1 and R. Karlsson1, 1Biacore AB, Uppsala, Sweden, 2Tohoku University, Sendai, Japan.
812 Determining the assay consistency for biosensor based direct binding assays
Annie Näslund, Christina Wass, Annika Remaeus and Åsa Edström, Dept. Systems and application, Biacore AB, Uppsala Sweden.
813 An acoustic sensor design based on a non-contact transducer format
Roque, A.C.A., Araya-Kleinsteuber, B., Kioupritzi, E., Stevenson, A.C., Lowe, C.R.
Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, UK CB2 1QT. Tel: +44 (0) 1223 334152 Fax: +44 (0) 1223 334162, email: car38@cam.ac.uk
814 Capacitive affinity biosensor as a tool to monitor low concentrations of
biomolecules

Martin Hedström, Igor Galaev and Bo Mattiasson, Department of Biotechnology, Center for Chemistry & Chemical Engineering, Lund University, P.O. Box 124, SE-22100 Lund, Sweden
815 Exploitation of the Janus-faced nature of ß-cyclodextrins as affinity ligands in CE and CEC
Ákos Végvári, Dept. Of Biochemistry, Uppsala University, P.O.Box 576, SE-751 23 Uppsala, Sweden
816 Affi body® Molecules: Affinity Proteins for Biotechnology Applications
Martin Nilsson and Thomas Bergman, Affibody AB, Voltavägen 13, 161 02 Bromma, Sweden
817 Polymeric IMAC-adsorbents and their use in the adsorption of Arsenic ions from
aqueous solutions

Javier E. Garcia*, Roberto Guzman* and Jerker Porath*+, *Chemical and Environmental Engineering Department, University of Arizona, Tucson, AZ.
+Centre for Separation Sciences, Uppsala University, Uppsala, Sweden.
818 Enhanced Affinity Gel Permeation Chromatography of Proteins Using a
Multiligand-Water Soluble Affinity Carrier

Javier E. Garcia*, Jerker Porath*+ and Roberto Guzman*, *Chemical and Environmental Engineering Department, University of Arizona, Tucson, AZ.
+Centre for Separation Sciences, Uppsala University, Uppsala, Sweden.
819 Dynamic Binding Capacities of Phosphorylase b on Experimental Butyl-Modified
Sepharose™ 4B, and Surface-Extended Sepharose 6 Critical Hydrophobicity HIC
Chromatography Media

Kristin Zurlinden1, James M. Van Alstine2 and Herbert P. Jennissen1, 1Institut für Physiologische Chemie, Universitätsklinikum Essen, Hufelandstr. 55, D-45122 Essen, Germany; 2GE Healthcare, Protein Separations, Uppsala, 112 54, Sweden
820 A New IMAC Medium for Scaling up Purification of Histidine-tagged Proteins
Jon Lundqvist, Lars C. Andersson, Ann Bergh, Ellinor Ancker, Anna Heijbel, Helena Lindgren, Karin Torstenson and Katarina Öberg, GE Healthcare, Protein Separations, SE-751 84 Uppsala, Sweden
821 Automated Multi-step Purification of Histidine-tagged proteins from Crude Cell Lysates
Anna Sjöberg, Ellinor Ancker, Ann Bergh, Jon Lundqvist, Katarina Öberg, Nina Forsberg
GE Healthcare, Bio-Sciences, Protein Separations, SE-751 84 Uppsala, Sweden
822 Hydrophilic Rigid Activated Media by Encapsulation of Inorganic Particles by
Dispersion Polymerization

M.M. Rhemrev-Boom and M.A. Jonker, ResQ lab B.V., Nijverheidsweg 14, 7948 NE Nijeveen, the Netherlands
823 Polymer-modulated permeation control for affi nity adsorption chromatography.
An Strategy to Enhance Peptide and Small Proteins Fractionations

Roberto Guzman*, Lian Wang*, and Jerker Porath*+, *Chemical and Environmental Engineering Department, University of Arizona, Tucson, AZ. +Centre for Separation Sciences, Uppsala University, Uppsala, Sweden.
824 Synthesis and Studies of Chelating Liposomes: Binding of Metal Ions and Proteins
and Effect of PEG on Aggregation and Binding Interactions

Shujuan Yan and Roberto Guzmán, Chemical and Environmental Engineering Department, University of Arizona, Tucson, AZ 85721
825

Selective adsorption of monoclonal antibodies against mutant amidase from
Pseudomonas aeruginosa on tailor-made immobilized metal chelates

Sónia Martins1, Jorge Andrade1, Amin Karmali*1 and Maria Luísa Serralheiro2, 1Centro de Investigação de Engenharia Química e Biotecnologia do Instituto Superior de Engenharia de Lisboa, Rua Conselheiro, Emídio Navarro 1900 Lisboa - Portugal, Tel: 351.21.8317052, Fax: 351.21.8317267, 2 Centro de Química e Bioquímica da Faculdade de Ciências da Universidade de Lisboa ,Campo Grande, 1760 Lisboa - Portugal. *To whom correspondence should be addressed. e-mail: akarmali@deq.isel.ipl.pt

826 AffilinTM– And Its Application In Affinity Chromatography
E. Fiedler, C. Reimann, M. Fiedler, T. Scheuermann, G. Proetzel, R. Rudolph, and U. Fiedler, Scil Proteins GmbH, Heinrich-Damerow-Str. 1, 06120 Halle/Saale, Germany
Tel.+49 345 27996330, Fax+49 345 27996332, http://www.scilproteins.com, e-mail: affi lin@scilproteins.com
827 Potential of homocysteine thiolactone scaffold towards libraries of media with new selectivies
Jean-Luc Maloisel, Nicolas Thevenin, Nils Norrman, Johanna Karlqvist, Miranda Varedian, GE Healthcare, Bjorkgatan 30, SE-751 84 Uppsala, Sweden
828 Affinity ligands for transferrin
Hörður Filippusson, University of Iceland Science Institute,Department of Biochemistry, Dunhaga 3, IS-107 Reykjavík, Iceland
829 Detection of weak antibody fragments using phage display
Eva Åström & Sten Ohlson, Department of Chemistry and Biomedical Sciences, University of Kalmar, SE-391 82 Kalmar, Sweden
830 Adsorption studies of recombinant cutinase onto a combinatorial library of
synthetic affinity ligands

Ruiu, L.1,2, Roque, A.C.A.1.2, Lowe, C.R.2, Taipa, M.A.1, 1Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Avenida Rovisco Pais, 1049-001 Lisboa, Portugal.
831 Development and applications of a new Protein A media intended for industrial use
Hans J Johansson, GE Healthcare, SE-751 84 Uppsala
832 Affinity cryogel monoliths for cell chromatography and cell surface profi ling
Maria B. Dainiaka,b, Fatima M. Plievaa,b, Igor Yu. Galaeva and Bo Mattiassona
a Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P-O. Box 124, SE-22100 Lund, Sweden, b Protista Biotechnology AB, IDEON, SE-22370 Lund, Sweden
833 An Optical Biosensor (SPR) Assay For Investigations of Enantioselective Affi nities
A Comparison with A Validated HPLC Method

Robert Arnell, Natalia Ferraz, Peter Sandblad and Torgny Fornstedt, Dept. of Surface Biotechnology, BMC Box 577, S-751 23 Uppsala, Sweden
834 Ion-exchange Macroporous Hydrophilic Gels with Grafted Polymer Brushes
Irina Savina, Igor Yu. Galaev and Bo Mattiasson, Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-22100 Lund, Sweden
835 Capture of Bacterial Endotoxins using a Supermacroporous Monolithic Matrix with
Immobilized Polyethyleneimine, Lysozyme or Polymyxin B

Amro Hanora1, Fatima M. Plieva2, Martin Hedström1, Igor Yu. Galaev1,2 and Bo Mattiasson1,2, 1Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-22100 Lund,, Sweden, 2Protista Biotechnology AB, IDEON, SE 223 70 Lund, Sweden
836 Ion Exchange Media for Separation Large Biomolecules
Jan Bergström*, Joachim Stadler+, Dag Lindström, Åsa Rudstedt, Robert Magnusson,
Rudolfo Kurt-Fuentes, James Van Alstine., GE Healthcare, Protein Separations, Uppsala, Sweden and +Freiburg, Germany
837 CBioSep – Swedish Centre for BioSeparation
Per-Olof Larsson, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden
838 Supermacroporous composite monolithic cryogels bearing immobilized metal
affi nity ligand

Fatima M. Plievaa,b, Maria-Rosa Aguilarc, Igor Yu. Galaeva, Bo Mattiassona,*
aDepartment of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-22100 Lund, Sweden, bProtista Biotechnology AB, IDEON, SE-22370 Lund, Sweden, cInstituto de Ciencia y Technologia de Polimeros, CSIC, C/Juan de la Cierva, 3, 28006 Madrid, Spain
839 Cryogels – supermacroporous gels for separation of particulate matter
Bo Mattiasson1, Igor Galaev1, Fatima Plieva2, Maria Dainiak2, 1Department of Biotechnology, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden, 2Protista Biotechnology AB, IDEON, SE 223 70 Lund, Sweden
840 Affi nity purification of proteins using quartz crystal microbalance sensor chip
Ermias Melles1, Henrik Anderson2,3, Daniel Wallinder2, Jawed Shafqat1, Tomas Bergman1, Teodor Aastrup2 and Hans Jörnvall1, 1Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden
2Attana AB, Björnnäsvägen 21, SE-113 47 Stockholm, Sweden, 3The Ångström Laboratory, Solid State Electronics, Uppsala University, P.O. Box 534, SE-751 21 Uppsala, Sweden
841 Escherichia coli K88 Interaction with Serum Porcine IgA Oligosaccharides
Gabriela Ramos-Clamont,* Roberto Guzman Z.‡ Joy Winzerling† and Luz Vázquez-Moreno*, *Centro de Investigación en Alimentación y Desarrollo A.C. Coordinación de Ciencia de los Alimentos. Apartado Postal 1735, Hermosillo, Sonora, México, 83000.,
‡ Department of Chemical & Environmental Engineering.,† Department of Nutritional Sciences, University of Arizona, Tucson, AZ 85721.
842 Membrane Recognition by the Cholesterol-Dependent Cytolysin Anthrolysin O
from Bacillus Anthracis

Simon Cocklin1, Stephen Weeks1, Noreen Robertson1, Monika Jost2, Hans-Walter Weber2, Emily Young1, Samarendra Seal3, Martin Zhang4, Patrick Loll1, Aleister Saunders4, Richard Rest3, and Irwin Chaiken1
1Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102, 2Department of Radiation Oncology, Drexel University College of Medicine, Philadelphia, PA 19102, 3Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19144,
4Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104
843 Metallizable, Site-Specific Coiled Coil Interfaces for Carbon Nanotube Arrays
Mark Contarino1,2, Mauro Sergi1, Adam Lazarak3, Syna Kuriakose1, Jimmy Xu3 and
Irwin Chaiken1, 1Dept. of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102. 2School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104. 3Division of Engineering and Dept. of Physics, Brown University, Providence, RI 02912.
844 Real-time protein interaction analysis in the development of an anti-IgE based
immunotherapeutic treatment for allergy and asthma

T Jarhede1, E Pol1, Å Edström1, M Fant3, G Franklin2, Å Frostell-Karlsson1, Å Jansson3, R Karlsson1, A Larsson1, S Persson3, 1System & Applications, Biacore AB 2Market Communication, Biacore AB 3Resistentia Pharmaceuticals AB
845 Design and Use of Affibody Molecules for Tumor Targeting
Tove Eriksson1, Joachim Feldwisch1, Anders Wennborg1, Stefan Ståhl2, Jörgen Carlsson3 and Fredrik Nilsson1, 1 Affi body AB, Box 20137, SE-161 02 Bromma, Sweden, www.affi body.com, 2 Royal Institute of Technology, Stockholm, Sweden
3 Institute of Biomedical Radiation Sciences, Uppsala, Sweden
846 Interaction of boronate-containing copolymers with saccharides, glycoproteins,
carbohydrate particles and cells

Alexander E. Ivanov, Marina V.Kuzimenkova, Igor Yu. Galaev, Bo Mattiasson
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, S-22100, Lund, Sweden
847 On the Double Nature of Chromatographic Peaks and Fronts: The Result of the
Propagation of a Mass and/or a Concentration Wave

Jörgen Samuelsson, Robert Arnell and Torgny Fornstedt, Dept. of Surface Biotechnology, BMC Box 577, SE-751 23 Uppsala, Sweden
848 Fast discovery of small molecule affinity ligands
Søren F. Christensen1, Henrik Rasmussen1, Monica Ramos1, Roice Michael1, Morten Meldal2, Ib Johannsen1*, 1 VersaMatrix A/S, Gamle Carlsberg Vej 4-6, DK-2500 Valby, Denmark, 2 Carlsberg Reseach Centre, Gamle Carlsberg Vej 10, DK-2500, Valby, Denmark
849 CD microlaboratories – a fully automated solution for fast and flexible IgG
quantifi cation in biopharmaceutical process development

Ebru Poulsen, Anders Hedström, Mats Inganäs, Gyros AB, Uppsala Science Park, SE-751 83 Uppsala, Sweden
850 Selective Separation of Cells: supermacroporous monolithic cryogel provide
a new tool

Ashok Kumar1,2, Hans Jungvid2 and Bo Mattiason1, 1Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, SE-22100 Lund, Sweden and 2Protista Biotechnology AB, Ideon Science Park, SE-22370 Lund, Sweden
851 A novel affinity based controlled release system involving derivatives of dextran
with enhanced osmotic activity for potential application in surgery

Jyothi Devakumar and M.A.Vijayalakshmi, LIMTech.S;Dept.of BioEngineering, Univ. Technol. De Compiegne, B.P.20529; 60205-Compiegne, France.
852 MAAS enables cost-effective affinity adsorption
Karin Merck1, Bert Dijkink1, Joyce Schroot1 and Jan-Henk Hanemaaijer2, 1Agrotechnology and Food Innovations B.V., Bornsesteeg 59, 6708 PD, Wageningen, The Netherlands, 2 TNO Milieu Energie en Procesinnovatie, Laan van Westenenk 501, 7300 AH Apeldoorn, The Netherlands
853 Conformational Entrapment of gp120: High Affinity Dual Antagonists through Click
Chemistry

Hosahudya Gopi1, Navid Madani2, Arne Schon3, Simon Cocklin1, Hui Xie4, Sabine Baxter1, Kalyan Tirupula1, Ernesto Freire3, Joseph Sodroski2, Wayne Hendrickson4, and Irwin Chaiken1
1Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102, 2Dana Farber Cancer Institute, Boston, MA 02115
3Department of Biology and Biophysics, The Johns Hopkins University, Baltimore, MD 21218, 4Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032
854 Cyanovirin-N-12p1, a Novel Chimeric Protein Inhibitor of HIV-1 Envelope
Interactions

Karyn McFadden, Hosahudya Gopi, Simon Cocklin and Irwin Chaiken, Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102
855 Use of Single Domain Heavy Chain Antibody Fragments as Ligands in Affinity
Chromatography

Pim Hermans, BAC b.v., Huizerstraatweg 28, NL-1411 GP Naarden, The Netherlands
856 The design and Synthesis of Carbohydrate Receptors
A. Hussain, M.C. Lee, J and C. R. Lowe*, Institute of Biotechnology, Tennis Court Road, Cambridge CB2 1QT
857 Receptor Epitope Usage by Interleukin 5 and its Antagonist Peptide
Tetsuya Ishino, Dominick Panarello, Udaya Pillalamarri, Madhushree Bhattacharya, Ceci Urbina, Stephanie Horvath, Noreen Robertson, Sanjay Sarkhel, Bradford Jameson and Irwin Chaiken
Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102, USA
858 Immobilisation of Ribonuclease I via Epoxy Groups or CDI Groups of
CIM Methacrylate Monoliths

M. Benčina1, J. Kuplenk1, A. Jungbauer2, A. Podgornik3, 1Laboratory of Biotechnology, National Institute of Chemistry, Hajdrihova 19, P.O.B. 600, SI-1001 Ljubljana, Slovenia, 2Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A-1190 Vienna, Austria, 3BIA Separations d.o.o., Teslova 30, SI-1001 Ljubljana, Slovenia